OBJECTIVE: The aim of the present study was to compare the surgical results with contrast-enhanced [99mTc]methoxy-isobutylisonitrile ([99mTc]MIBI) single-photon emission computed tomography (SPECT)/computed tomography (CT) results by adding the simultaneous single phase contrast to the [99mTc]MIBI SPECT/CT examination. METHOD: Between November 2016 and January 2018, 24 (21 female, 3 male) patients who underwent an operation after preoperative contrast-enhanced [99mTc]MIBI SPECT/CT, and 49 asymptomatic patients (42 female, 7 male), who did not undergo surgery, were included in the study. RESULTS: Sensitivity and specificity rates of contrast enhanced [99mTc]MIBI SPECT/CT were 100% and 100% when evaluated together with surgical results. The rate of detection in asymptomatic patients was found to be 93.8%. CONCLUSIONS: With the contrast-enhanced [99mTc]MIBI SPECT/CT imaging, both high sensitivity and specificity val- ues were achieved in the preoperative localization of parathyroid adenomas (PTAs) and high detection rates were obtained in the asymptomatic group. The use of contrast IV during the [99mTc]MIBI SPECT/CT in patients without con- traindications increased both preoperative localization rates of adenomas and detection rates of asymptomatic hyper- parathyroidism cases.