INTRODUCTION: Situs Viscerum Inversus totalis (SIT) is a rare anomaly with genetic predisposition, in which organs are translated, completely or partially, on the opposite side of the body. Generally there are no organic dysfunctions. Situs Inversus can cause difficulties in the diagnostic and therapeutic management of abdominal diseases because of the mirror-like anatomy. On a clinical point of view the symptoms of cholelithiasis may be confused by the opposite position of the gall bladder CASE PRESENTATION: We report the case of a 48 year old female latin-american with symptomatic cholelithiasis and Situs Viscerum Inversus Totalis, treated with Laparoscopic Cholecystectomy. CONCLUSION: Videolaparoscopy represents the gold standard treatment in managing cholelithiasis in SIT patients. Surgical treatment can be facilitated in case of well-experienced operators, as it is well recognised a major difficulty for surgeons in managing the anatomical condition of SIT