The protective effect of melatonin on remote organ liver ischemia and reperfusion injury following aortic clamping


COD: 03_2016_271-280 Categorie: ,

Mustafa Ozsoy, Yucel Gonul, Ziya Taner Ozkececi, Ahmet Bal, Ruchan Bahadir Celep, Ahmet Koçak, Fahri Adali, Murat Tosun, Sefa Celik

Ann. Ital. Chir., 2015 86: 271-279

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BACKGROUND: Severe local and systemic tissue injuries can occur after restoration of tissue oxygenation which is also
known as reperfusion injury. Our objective was to investigate the possible protective effects of melatonin against IR damage
in hepatic tissue following infrarenal aortic occlusion.
METHODS: A total of twenty-one male Wistar-albino rats separated into three groups as follows: Group I: Laparotomy
and dissection of the infrarenal abdominal aorta (AA) were concurrently performed. Group II: About 1 ml of 0.9% saline
was intraperitoenally administered 30 min before and after the occlusion operation. After laparotomy and dissection,
infrarenal AA was clamped for 30 minutes and then was exposed to two hours of reperfusion. Group III: The melatonin
was administered 30 min before clamping of the infrarenal AA then 30 min of ischemia and two hours of reperfusion
was applied.
RESULTS: Serum aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase levels were remarkably
higher in IR group, when compared with the sham group, and the laboratory tests returned to normal levels in IR+MEL
group after treatment. Although serum IL-1β, IL-6, IL-18, TNF-α, and IFN- γ levels have decreased in treatment
group following melatonin administration, this decrement was statistically significant for serum IL-18, TNF-α, and IFN-
γ parameters compared with the IR group. Serum levels of TOC and OSI were decreased and tissue levels of TAC were
increased by melatonin.
CONCLUSION: As a result of this study, it can be suggested that melatonin has antioxidant, anti-inflammatory and hepatoprotective
effects in case of IR.