CELL-MEDIATED IMMUNITY, AFTER LAPAROSCOPIC CHOLECYSTECTOMY

Abstract

Cell - mediated immunity (CMI) is temporarily decreased after major surgical procedures in relation to type of anaesthesia, lenght and severity of an intervention. In this study it has been evaluated CMI response in vivo, after laparoscopic (LC) and open cholecystectomy (OC).

5 healthy persons and 16 patients with symptomatic and uncomplicated gallstones were studied. They were subdivided in two groups: 8 were treated by OC, 8 by LC. A skin test system (Multitest, IMC - Institute Merieux, Lyon, France ) consisting of a plastic disposable multiple-puncture device capable of simultaneous application of seven delayed-type hypersensitivity antigens and a glycerin and saline diluent (negative control) was used. The test was performed 48 hours before and 24 hours after the surgical procedure. A diameter of 2 mm or more of the induration was considered as a positive response. A score expressed ,as the sum in millimeters of all the mean diameters (DM) of positive reactions and as numbers of antigens (NA) positive was calculated. Statistics was assessed by Student’s t test for comparison of averages.

There was no difference of age, sex, anasthesiologic risk, lenght of the operation between the two groups. We observed a significative reduction of DM (P < 0,05) and of NA (P < 0,05) in patients in which OC was performed while there were no significant in the group treated by LC.

Results show a significant suppression of the CMI after OC, no particular changes after LC according to other studies in vitro.