Identification of prognosis-related proteins in gingival squamous cell carcinoma by twodimensional gel electrophoresis and mass spectrometry-based proteomics

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COD: 02_1016_2258 Categorie: ,

Maria Giulia Cristofaro, Domenica Scumaci, Claudia Vincenza Fiumara, Maddalena Di Sanzo, Valeria Zuccalà, Giuseppe Donato, Davide Caruso, Umberto Riccelli, Maria Concetta Faniello, Giovanni Cuda, Francesco Costanzo, Mario Giudice

Ann. Ital. Chir., 2014 85: 518-524

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OBJECTIVES: The aim of this study was to identify differentially expressed proteins in oral squamous carcinoma cells that
could be potential prognosis-related cancer biomarkers.
MATERIALS AND METHODS: We compared protein expression patterns from gingival squamous cellc carcinoma (GSCC)
tissues and adjacent non-cancerous matched tissues by proteomic analysis using two-dimensional gel electrophoresis coupled
to mass spectrometry (2D-PAGE/MS).
RESULTS: Seventeen protein spots were found to be over-expressed and eight were under-expressed in cancerous tissue compared
to the normal counterpart. Of these, annexin A2 and ezrin were validated by Western blot. We also demonstrated
by immunohistochemistry that POSTN is highly expressed in the neoplastic tissues examined. Among the differentially
expressed proteins, we focused our attention on Chloride intracellular channel 1 (CLIC1).
CONCLUSION: The 2D-PAGE/MS-based proteomics appears an efficient approach in detecting and identifying differentially
expressed proteins that might function as potential biomarkers and/or molecular targets for early cancer diagnosis
and prognosis and that might contribute to a innovative therapeutic strategies in GSCC. However, further validation
and functional studies are needed to confirm and to support these promising, still preliminary data.

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