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We define as Banking of the tissues all the procedures that
include the finding, preparation, conservation and
distribution of the homograft.
The vascular homografts are taken and put into a solution
of trasportation at + 4°C and kept at this temperature till their arrival at the Bank. The following step is the dissection
of the homograft which will have to be performed as quickly
as possible at most 24 hours after the taking in conditions
of maximum sterility.
At the Italian Homograft Bank at Centro Cardiologico, the
vascular homografts are kept at + 4°C for 96 hours on
average with antibiotics. After a phase of sterilization at +
4°C the tissue is frozen according to a homogeneus and
controlled thermic decrease and stored at –150°C/-180°C in
fumes of liquid nitrogen till the moment of their employment
allowing a long term conservation.
The aim of all these procedures of cryopreservation is to keep
the structural and functional integrity of cells and tissues.
The thermic decrease of the tissues must occur so that to avoid
all the damages of the cellular vitality and functionality and
especially of the tissue structure in toto.
In order to limitate these events some cryoprotector agents are
employed because they reduce the concentration of the solutes,
the cellular dehydratation, the formation of micro-macro
Another step to establish if the homograft is proper is the
study of bacteriological and viral aspects. The viral screenings
are performed on the donor’s blood and the bacteriological
tests are performed on the tissue and on the liquids.
For each phase of the banking a series of information about
the donor and about the tissues are recorded and filed both
on paper and database so that to grant always a right conduct
of the material.